stringtie - 2.2.1+ds-2 main

The abundance of transcripts in a human tissue sample
can be determined by RNA sequencing. The exact sequence
sampled may be random, depending on the technology used.
And it may be short, i.e. shorter than the transcript.
At some point, many shorter reads need to be assembled
to the model the complete transcripts.
.
StringTie knows how to assemble of RNA-Seq into potential
transcripts without the need of a reference genome and
provides a quantification also of the splice variants.