ruby-crb-blast - 0.6.9-5 main

CRB-BLAST is a novel method for finding orthologs between one set of sequences
and another. This is particularly useful in genome and transcriptome
annotation.
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CRB-BLAST initially performs a standard reciprocal best BLAST. It does this by
performing BLAST alignments of query->target and target->query. Reciprocal
best BLAST hits are those where the best match for any given query sequence in
the query->target alignment is also the best hit of the match in the reverse
(target->query) alignment.
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Reciprocal best BLAST is a very conservative way to assign orthologs. The main
innovation in CRB-BLAST is to learn an appropriate e-value cutoff to apply to
each pairwise alignment by taking into account the overall relatedness of the
two datasets being compared. This is done by fitting a function to the
distribution of alignment e-values over sequence lengths. The function
provides the e-value cutoff for a sequence of given length.